Journal: Scientific Reports

Article Title: Various distribution of BMPs in different periosteal layers contributing to inconsistent osteoinductivity of DBM-based products

doi: 10.1038/s41598-026-35269-z

Figure Lengend Snippet: The amount of BMP-2 in DBM significantly increased in the order of OD > MD > ID using either extracted method, and was more than cortical particles.

Article Snippet: Sandwich enzyme-linked immunosorbent assay (ELISA) was used to assess the amounts of BMP with Commercially available quantikine kits purchased from MULTI SCIENCES (LIANKE) BIOTECH, CO, LTD (human BMP-2 ELISA Kit, sensitivity, 1.73 pg/ml; BMP-7 ELISA Kit, sensitivity, 2.06 pg/ml).

Techniques:

Journal: Scientific Reports

Article Title: Various distribution of BMPs in different periosteal layers contributing to inconsistent osteoinductivity of DBM-based products

doi: 10.1038/s41598-026-35269-z

Figure Lengend Snippet: The trend of BMP-7 distributed in DBM and cortical particles was similar with BMP-2.

Article Snippet: Sandwich enzyme-linked immunosorbent assay (ELISA) was used to assess the amounts of BMP with Commercially available quantikine kits purchased from MULTI SCIENCES (LIANKE) BIOTECH, CO, LTD (human BMP-2 ELISA Kit, sensitivity, 1.73 pg/ml; BMP-7 ELISA Kit, sensitivity, 2.06 pg/ml).

Techniques:

Journal: Scientific Reports

Article Title: Various distribution of BMPs in different periosteal layers contributing to inconsistent osteoinductivity of DBM-based products

doi: 10.1038/s41598-026-35269-z

Figure Lengend Snippet: Through the linear regression analysis, the content of BMP-7 varied linearly with the content of BMP-2 (GuHCl vs. collagenase, R2 = 0.945 vs. R2 = 0.818, respectively).

Article Snippet: Sandwich enzyme-linked immunosorbent assay (ELISA) was used to assess the amounts of BMP with Commercially available quantikine kits purchased from MULTI SCIENCES (LIANKE) BIOTECH, CO, LTD (human BMP-2 ELISA Kit, sensitivity, 1.73 pg/ml; BMP-7 ELISA Kit, sensitivity, 2.06 pg/ml).

Techniques:

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Preparation and in vitro sustained release performance of thermosensitive gel microsphere composite loaded with Anti-Tuberculosis drug PaMZ/BMP-2

doi: 10.1186/s13018-025-06595-1

Figure Lengend Snippet: Flowchart of PaMZ/BMP-2 microsphere preparation process. The outer phase was prepared by dissolving 0.4 g of polyvinyl alcohol in 20 mL of ultrapure water with stirring at 70 °C until completely dissolved, then slowly cooled to obtain a 2% PVA solution. The inner phase was formed by mixing the inner aqueous phase—comprising 4 mg Moxifloxacin, 15 mg Pyrazinamide, and 1 μg BMP-2 dissolved in 1 mL ultrapure water via 5-min ultrasonication—with the oil phase, which contained 1 mg Pretomanid and 46.67 mg PLGA dissolved in 2.5 mL dichloromethane by 3-min ultrasonication. The two phases were combined by vortex mixing followed by 5 min ultrasonication to form the inner phase liquid. Finally, the PaMZ/BMP-2 thermosensitive gel microsphere composite was constructed using microfluidic technology by combining the inner and outer phases, ensuring high drug loading and controlled release

Article Snippet: Internal aqueous phase (M/Z/BMP-2 solution) 10 μg of BMP-2 (HY-P7006, MedChemExpress) was dissolved in 10 μL of acetic acid (HAc) as a stock solution.

Techniques: Construct

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Preparation and in vitro sustained release performance of thermosensitive gel microsphere composite loaded with Anti-Tuberculosis drug PaMZ/BMP-2

doi: 10.1186/s13018-025-06595-1

Figure Lengend Snippet: Morphological characterization diagram of PaMZ/BMP-2 microspheres. A Shows the macroscopic appearance of the microspheres observed with the naked eye. B Presents the morphology of the microspheres captured by a computer terminal during the preparation process. C Displays a 400 × magnified image under an optical microscope. D Shows the surface morphology of drug-loaded microspheres after drying and gold sputtering, as observed by field emission scanning electron microscopy

Article Snippet: Internal aqueous phase (M/Z/BMP-2 solution) 10 μg of BMP-2 (HY-P7006, MedChemExpress) was dissolved in 10 μL of acetic acid (HAc) as a stock solution.

Techniques: Microscopy, Electron Microscopy

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Preparation and in vitro sustained release performance of thermosensitive gel microsphere composite loaded with Anti-Tuberculosis drug PaMZ/BMP-2

doi: 10.1186/s13018-025-06595-1

Figure Lengend Snippet: Particle size distribution map of PaMZ/BMP-2 microspheres

Article Snippet: Internal aqueous phase (M/Z/BMP-2 solution) 10 μg of BMP-2 (HY-P7006, MedChemExpress) was dissolved in 10 μL of acetic acid (HAc) as a stock solution.

Techniques:

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Preparation and in vitro sustained release performance of thermosensitive gel microsphere composite loaded with Anti-Tuberculosis drug PaMZ/BMP-2

doi: 10.1186/s13018-025-06595-1

Figure Lengend Snippet: X-ray diffraction intensity—diffraction Angle curve of PaMZ/BMP-2 microspheres

Article Snippet: Internal aqueous phase (M/Z/BMP-2 solution) 10 μg of BMP-2 (HY-P7006, MedChemExpress) was dissolved in 10 μL of acetic acid (HAc) as a stock solution.

Techniques: